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PCR Mastercycler ep realplex2 Excitation source 96 LEDs ( 470 nm)
Emission filters 520 / 550 nm ( realplex2)
520 / 550 / 580 / 605 nm ( realplex4)
Detector 1-channel photomultiplier tube ( realplex2)
2-channel photomultiplier tubes ( realplex4)
Dynamic range 9 orders of magnitude from starting copy number
Sensitivity d 50 fmol fluorescein
Thermo module
Sample capacity 96 x 0.2 ml PCR tubes or one 96 PCR plate
( unskirted, semi-skirted, skirted— as per SBS standard)
Temperature control range of block 4– 99 ° C
Degree range of gradient, maximum 1– 20 ° C ( thermomodule Mastercycler ep)
1– 24 ° C ( thermomodule Mastercycler ep S)
Temperature control range of gradient 30– 99 ° C
Temperature of lid 105 ° C
Block homogeneity 20– 72 ° C ± 0.3 ° C
90 ° C ± 0.4 ° C
Control accuracy ± 0.2 ° C
Heating speed* approx. 4 ° C/ s ( thermomodule Mastercycler ep)
approx. 6 ° C/ s ( thermomodule Mastercycler ep S)
Cooling speed* approx. 3 ° C/ s ( thermomodule Mastercycler ep)
approx. 4.5 ° C/ s ( thermomodule Mastercycler ep S)
Complete system
Dimensions ( W x D x H) 26 cm x 41 cm x 39.6 cm
Total weight ( detection module) 24 kg ( 7 kg)
Voltage requirements 200– 240 V, 50 Hz– 60 Hz
Power consumption 800 W
* Measured at block
Mastercycler® ep realplex
The Mastercycler ep realplex is currently not available in
every country. For further information please contact your
local Eppendorf organisation.
Ordering information
Description Order no.
Mastercycler® ep realplex:
Mastercycler® ep realplex2, with aluminum block and two emission filters 6300 000.507
Mastercycler® ep realplex2 S, with silver block and two emission filters 6300 000.604
Mastercycler® ep realplex4, with aluminum block and four emission filters 6302 000.504
Mastercycler® ep realplex4 S, with silver block and four emission filters 6302 000.601
Optical detection module to upgrade Mastercyler ep gradient/ S
realplex2 6300 000.000
realplex4 6302 000.008
System accessories
twin.tec PCR Plates, see page 199 or visit www.eppendorf.com
Service
Preventive maintenance contracts, see page 191
Special cycler versions
Mastercycler® ep realplex2 i 6300 000.957
Mastercycler® ep realplex4 i 6302 000.954
Technical specifications subject to change. Ordering information for other voltage options
can be found in the appendix on page 304.